ICC shows positive staining in HeLa cells. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/2000 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
p16 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-303-206)
p16 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-303-206)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p16 |
| Synonyms | Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A (CDK4I), Multiple tumor suppressor 1 (MTS-1), p16-INK4a (p16-INK4; p16INK4A), CDKN2A, CDKN2, MTS1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P42771 |
| Clone Number | SDT-303-206 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500-1:2000 | |
| IF | 1:200-1:1000 |
Background
p16 (also known as p16INK4a, cyclin-dependent kinase inhibitor 2A, CDKN2A, multiple tumor suppressor 1 and numerous other synonyms), is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby acting as a tumor suppressor. p16 can be used as a biomarker to improve the histological diagnostic accuracy of grade 3 cervical intraepithelial neoplasia (CIN). p16 is also implicated in the prevention of melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, vulvar cancer and esophageal cancer.
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Immunocytochemistry
Negative control：ICC shows negative staining in MCF7 cells. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/2000 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Immunofluorescence
IF shows positive staining in paraffin-embedded Human cervical squamous cell carcinoma. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
