IHC shows positive staining in paraffin-embedded human lung. Anti-NaPi2b/SLC34A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
NaPi2b/SLC34A2 Recombinant Rabbit mAb, PBS Only (SDT-1771-79)
NaPi2b/SLC34A2 Recombinant Rabbit mAb, PBS Only (SDT-1771-79)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NaPi2b/SLC34A2 |
| Synonyms | Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na(+)-dependent phosphate cotransporter 2B; NaPi3b; Sodium/phosphate cotransporter 2B (Na(+)/Pi cotransporter 2B; NaPi-2b); Solute carrier family 34 member 2 |
| Immunogen | Synthetic Peptide |
| Location | Apical cell membrane |
| Accession | O95436 |
| Clone Number | SDT-1771-79 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
| IF | 1:1000 | Hu |
Background
NaPi2b, also known as SLC34A2, is a sodium-dependent phosphate transporter encoded by the SLC34A2 gene and is a member of the SLC34 family. It is an eight-transmembrane protein that plays a crucial role in phosphate homeostasis by facilitating the trans-epithelial transport of phosphate. NaPi2b is highly expressed in various cancers, including non-small cell lung cancer (NSCLC), ovarian cancer, and papillary thyroid carcinoma, making it a promising target for cancer diagnosis and therapy. In normal tissues, its expression is limited, which further highlights its potential as a therapeutic target. Functionally, NaPi2b is involved in dietary phosphate absorption in the small intestine and surfactant synthesis in lung alveoli.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-NaPi2b/SLC34A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-NaPi2b/SLC34A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-NaPi2b/SLC34A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-NaPi2b/SLC34A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-NaPi2b/SLC34A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti- NaPi2b/SLC34A2 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
