The RAF1[Y340D/Y341D] activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the RAF1[Y340D/Y341D] protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Accession | P04049-1 |
Amino Acid Sequence | S306-F648(end),N-terminal GST tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 65568.0 |
Purity | >70% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 25mM Hepes, 300mM NaCl, 1mM DTT, 10% glycerol, 0.05% Brij-35, pH7.5 |
Stability & Storage | -80℃ |
Picture
Picture
Bioactivity

SDS-PAGE

M MW Standard
1 1 μg RAF1[Y340D/Y341D]

