The RAF1[Y340D/Y341D] activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the RAF1[Y340D/Y341D] protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P04049-1 |
| Amino Acid Sequence | S306-F648(end),N-terminal GST tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 65568.0 |
| Purity | >70% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 25mM Hepes, 300mM NaCl, 1mM DTT, 10% glycerol, 0.05% Brij-35, pH7.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity

SDS-PAGE

M MW Standard
1 1 μg RAF1[Y340D/Y341D]
