IKAK4 titration in ADP-Glo assay
Product Details
Product Details
Product Specification
Species | Human |
Accession | Q9NWZ3-1 |
Amino Acid Sequence | M1-S460(end), N-terminal GST tag, C-terminal Strep II tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 78.5 kDa |
Purity | >95% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Tag | GST Tag, Strep II tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, pH7.5 |
Stability & Storage | -80℃ |
Picture
Picture
Bioactivity


The IKAK4 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the IRAK4 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
SDS-PAGE



