IKAK4 titration in ADP-Glo assay
IRAK4 GST&Strep II Tag Protein, Human
IRAK4 GST&Strep II Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | Q9NWZ3-1 |
| Amino Acid Sequence | M1-S460(end), N-terminal GST tag, C-terminal Strep II tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 78.5 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag, Strep II tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, pH7.5 |
| Stability & Storage | -80℃ |
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Bioactivity
The IKAK4 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the IRAK4 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
SDS-PAGE
