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FeLV P27, His tag

FeLV P27, His tag

Catalog Number: S0A9042 Brand: Starter
Price:
Regular price $65.00 SGD
Regular price Sale price $65.00 SGD
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Product Details

Product Specification


Species FeLV
Synonyms Core polyprotein
Accession P10262
Amino Acid Sequence

Protein sequence (P10262, Pro198-Leu445, with C-10*His) PLREGPNNRPQYWPFSASDLYNWKSHNPPFSQDPVALTNLIESILVTHQPTWDDCQQLLQALLTGEERQRVLLEARKQVPGEDGRPTQLPNVIDETFPLTRPNWDFATPAGREHLRLYRQLLLAGLRGAARRPTNLAQVKQVVQGKEETPAAFLERLKEAYRMYTPYDPEDPGQAASVILSFIYQSSPDIRNKLQRLEGLQGFTLSDLLKEAEKIYNKRETPEEREERLWQRQEERDKKRHKEMTKVLGGGGSHHHHHHHHHH

Expression System E.coli
Molecular Weight Predicted MW: 30.7 kDa Observed MW: 30.7 kDa
Purity >95% by SDS-PAGE
Endotoxin <1EU/μg
Tag with C-10*His
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.05M Tris-HCl, pH8.0.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

Background

FeLV is an oncogenic gammaretrovirus. FeLV is identified as the infectious agent causing immunomodulation within bone marrow and the immune system, which renders infected cats susceptible to a variety of secondary and opportunistic infections. Associated diseases of FeLV include; lymphoma, non-regenerative anemias and thymic degenerative disease. Accurate blood testing procedures revolving around the detection of FeLV P27 enables diagnosis via two methods; enzyme-linked immunosorbent assay (ELISA), which detects the presence of free FeLV particles that are found in the bloodstream and indirect immunofluorescent antibody assay (IFA), which detects the presence of retroviral particles within white blood cells.

Picture

SDS-PAGE

2μg(R: reducing conditions)

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