EPHA3 titration in HTRF assay
EPHA3 Protein
EPHA3 Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P29320-1 |
| Amino Acid Sequence |
K579-V983(end) KRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNVDITTFRTTGDWLNGVWTAHCKEIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIISSIKALETQSKNGPVPV |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 71.9 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, 1mM DTT, 10% Glycerol, 0.05% Briji-35, pH7.5 |
| Stability & Storage |
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws
cycles.
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Bioactivity
The EPHA3 activity was detected using homogeneous time-resolved fluorescence technology. The reaction was performed by incubating the EPHA3 protein , ATP and substrate at 25℃ for 50 min, adding the TK-mix at 25℃ for 60 min, then reading ratio 665/620 nm with BMG.
SDS-PAGE
SEC-HPLC
