2μg (R: reducing condition, N: non-reducing condition).
Biotinylated CD5 His&Avi Tag Protein, Human
Biotinylated CD5 His&Avi Tag Protein, Human
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$636.00 SGD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD5; LEU1 |
| Accession | P06127-1 |
| Amino Acid Sequence | Arg25-Asn371, with C-terminal His and Avitag |
| Expression System | HEK293 |
| Molecular Weight | 53-60kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Bhandoola A, Bosselut R, Yu Q, Cowan ML, Feigenbaum L, Love PE, et al.. CD5-Mediated Inhibition of TCR Signaling During Intrathymic Selection and Development Does Not Require the CD5 Extracellular Domain. Eur J Immunol (2002) 32:1811. |
Background
CD5, one of the earliest markers used to identify T cells, is a 67 kD transmembrane molecule that is constitutively expressed on all T cells and is a negative regulator of lymphocyte function. T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1. CD5 is also a negative regulator of thymus cell stimulation. Lack of CD5 promotes positive selection of poorly selected thymocytes and increases negative selection of high-affinity clones. Along with its negative effect on T cell stimulation, CD5 was shown to diminish activation-induced cell death (AICD) through its interaction with casein kinase 2 (CK2).
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SDS-PAGE
ELISA
Immobilized Biotinylated CD5 His&Avi Tag Protein, Human (Cat. No. UA010808) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind CD5 Mouse mAb (S-576-35) with EC50 of 4.01-5.72ng/ml.
