WB result of TMEM119 Rabbit mAb
Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 55 kDa
(This blot was developed with high sensitivity substrate)
TMEM119 Recombinant Rabbit mAb (S-838-46)
TMEM119 Recombinant Rabbit mAb (S-838-46)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Transmembrane protein 119, Osteoblast induction factor (OBIF) |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cell membrane |
| Accession | Q8R138 |
| Clone Number | S-838-46 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| ICC | 1:500 | null |
| ICFCM | 1:500 | null |
Background
TMEM119 is a type I transmembrane protein originally identified as a regulator of osteoblast differentiation, which is expressed in several tissues. In the brain, TMEM119 is specific to microglia, and although its role is still unknown, could represent a useful tool to differentiate resident microglia from macrophages. TMEM119 plays an important role in bone formation and normal bone mineralization.
Picture
Picture
Western Blot
WB result of TMEM119 Rabbit mAb
Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 55 kDa
(This blot was developed with high sensitivity substrate)
WB result of TMEM119 Rabbit mAb
Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 55 kDa
(This blot was developed with high sensitivity substrate)
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling TMEM119 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells. Anti-TMEM119 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
