WB result of Synapsin-1 Rabbit mAb
Primary antibody: Synapsin-1 Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74, 70 kDa
Observed MW: 77, 68 kDa
Synapsin-1 Recombinant Rabbit mAb (S-550-7)
Synapsin-1 Recombinant Rabbit mAb (S-550-7)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Synapsin-1 |
| Synonyms | Brain protein 4.1, Synapsin I, SYN1 |
| Immunogen | Synthetic Peptide |
| Accession | P17600 |
| Clone Number | S-550-7 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC | 1:2000 |
Background
The synapsin I protein is a member of the synapsin family that are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Pathways leading to the induction of human eS6 phosphorylation have been found to enhance IL-8 protein synthesis. This mechanism is dependent on A/U-rich proximal sequences (APS) found in the 3'UTR of IL-8 immediately after the stop codon. Synapsin I plays important roles in synapse formation where they specifically minimize depletion of the neurotransmitter at the inhibitory synapses by contributing to the anchoring of synaptic vesicles.
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Western Blot
WB result of Synapsin-1 Rabbit mAb
Primary antibody: Synapsin-1 Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse lung lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74, 70 kDa
Observed MW: 77, 68 kDa
IP
Synapsin-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Synapsin-1 in 0.4 mg mouse brain lysate.
Western blot was performed on the immunoprecipitate using Synapsin-1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: mouse brain lysate 20 µg (Input)
Lane 2: Synapsin-1 Rabbit mAb IP in mouse brain lysate
Lane 3: Rabbit monoclonal IgG IP in mouse brain lysate
Predicted MW: 74, 70 kDa
Observed MW: 77,68 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human gastric cancer. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse lung. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-Synapsin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
