WB result of SH-PTP2 Recombinant Rabbit mAb
Primary antibody: SH-PTP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
SH-PTP2 Recombinant Rabbit mAb (S-1318-2)
SH-PTP2 Recombinant Rabbit mAb (S-1318-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SH-PTP2 |
| Synonyms | Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D (PTP-1D); Protein-tyrosine phosphatase 2C (PTP-2C); SHP-2; Shp2; SH-PTP3; PTPN11; PTP2C; SHPTP2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q06124 |
| Clone Number | S-1318-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, 293T, Jurkat, NIH/3T3, C6 |
| Predicted Reactivity | Ck |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
SH-PTP2, also known as SHP-2, is a non-receptor type protein tyrosine phosphatase (PTPN11) that contains two Src homology 2 (SH2) domains and a phosphatase domain. SHP-2 plays a crucial role in various cellular signaling pathways, including those downstream of growth factors, cytokines, and adhesion receptors. It modulates specific protein-protein interactions by binding to phosphotyrosine residues through its SH2 domains. Under normal conditions, the catalytic efficiency of SHP-2 is low due to the autoinhibition of its SH2 domains, but its catalytic activity increases when the SH2 domains are occupied by phosphotyrosine residues, possibly by inducing conformational changes in the enzyme. SHP-2 is involved in multiple signaling pathways, including the Ras-Raf-MAP kinase, Jak-Stat, and PI3 kinase pathways, and may function at multiple sites within a single signaling pathway. SHP-2 is also associated with a variety of diseases, including Noonan syndrome, LEOPARD syndrome, juvenile myelomonocytic leukemia (JMML), and cartilage development abnormalities. Gain-of-function mutations in SHP-2 lead to developmental disorders such as Noonan syndrome and are frequently found in JMML patients. Additionally, SHP-2 has antitumor activity in various cancer models, and its inhibitors are being investigated as potential therapeutic agents for cancer treatment.
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Western Blot
WB result of SH-PTP2 Recombinant Rabbit mAb
Primary antibody: SH-PTP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
WB result of SH-PTP2 Recombinant Rabbit mAb
Primary antibody: SH-PTP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
IP
SH-PTP2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SH-PTP2 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using SH-PTP2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: SH-PTP2 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 68 kDa
Observed MW: 68 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-SH-PTP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-SH-PTP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SH-PTP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SH-PTP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-SH-PTP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-SH-PTP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- SH-PTP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SH-PTP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
