WB result of Sarcomeric Alpha Actinin Recombinant Rabbit mAb
Primary antibody: Sarcomeric Alpha Actinin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 104 kDa
Observed MW: 110 kDa
Sarcomeric Alpha Actinin Recombinant Rabbit mAb (S-1652-40)
Sarcomeric Alpha Actinin Recombinant Rabbit mAb (S-1652-40)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Sarcomeric Alpha Actinin |
| Synonyms | Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P35609 |
| Clone Number | S-1652-40 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | C2C12, mouse heart, rat heart, rat skeletal muscle |
| Predicted Reactivity | Bv, Ck, Mq |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Ms |
Background
Sarcomeric Alpha Actinin belongs to the spectrin superfamily, which includes spectrin, dystrophin, and their homologues and isoforms. It forms an anti-parallel rod-shaped dimer with one actin-binding domain at each end, bundling actin filaments in various cell types and cytoskeleton frameworks. In striated, cardiac, and smooth muscle cells, Sarcomeric Alpha Actinin is localized at the Z-disk and analogous dense bodies, where it forms a lattice-like structure and stabilizes the muscle contractile apparatus. Alpha Actinin functions as an actin crosslinker in muscle cells, anchoring actin filaments at the Z lines in striated muscles and dense bodies in smooth muscle cells. It plays a role in muscle contraction by interacting with the contractile machinery. In non-muscle cells, Sarcomeric Alpha Actinin is found along actin filaments and in adhesion sites, where it contributes to the organization of the cytoskeleton and cell adhesion. Sarcomeric Alpha Actinin is also implicated in autoimmune diseases, particularly systemic lupus erythematosus and autoimmune hepatitis, where it may serve as a target autoantigen.
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Western Blot
WB result of Sarcomeric Alpha Actinin Recombinant Rabbit mAb
Primary antibody: Sarcomeric Alpha Actinin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 104 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse heart. Anti-Sarcomeric Alpha Actinin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
