WB result of S100A4 Recombinant Rabbit mAb
Primary antibody: S100A4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: A375 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 12 kDa
Observed MW: 12 kDa
S100A4 Recombinant Rabbit mAb (S-1655-26)
S100A4 Recombinant Rabbit mAb (S-1655-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | S100A4 |
| Synonyms | Protein S100-A4; Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4; CAPL; MTS1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P26447 |
| Clone Number | S-1655-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, A549, A375, NIH/3T3 |
| Predicted Reactivity | Bv, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu, Ms |
Background
S100A4 is a member of the S100 protein family and is a calcium-binding protein with an EF double helix domain. It promotes the metabolic reprogramming of M2-type tumor-associated macrophages (TAMs) by enhancing the utilization of extracellular fatty acids and increasing mitochondrial-dependent fatty acid oxidation (FAO) levels. This controls the transformation of M2-type TAMs towards a "pro-tumor" phenotype. Deletion of S100A4 in mice leads to a significant reduction in the proportion of M2-type TAMs in the tumor microenvironment (TME) and inhibits tumor growth. S100A4 is significantly upregulated in various inflammatory cells such as T lymphocytes, dendritic cells, and macrophages in the dermis of psoriatic skin compared to healthy skin, indicating its role as an active inflammatory cytokine in psoriasis pathogenesis.
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Western Blot
WB result of S100A4 Recombinant Rabbit mAb
Primary antibody: S100A4 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 12 kDa
Observed MW: 12 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-S100A4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-S100A4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-S100A4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A375 cells. Anti- S100A4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- S100A4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
