IHC shows positive staining in paraffin-embedded mouse cerebral cortex (T cells). Anti-CD4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
S-RMab®CD4 Recombinant Rabbit mAb (S-R569)
S-RMab®CD4 Recombinant Rabbit mAb (S-R569)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD4 |
| Synonyms | T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3 |
| Location | Cell membrane |
| Accession | P06332 |
| Clone Number | S-R569 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250 | Ms |
| IF | 1:100 | Ms |
Background
CD4 is a glycoprotein found on the surface of immune cells, primarily known for its role as a coreceptor on T-helper cells, a subset of T lymphocytes. CD4 is used as a marker to distinguish between different T cell subsets, particularly T-helper cells (CD4+ T cells) and T-regulatory cells (also known as Tregs, which can be CD4+CD25+). CD4 acts as a coreceptor with the T cell receptor (TCR) to recognize antigens presented on the surface of antigen-presenting cells (APCs) by MHC class II molecules. This interaction is crucial for the activation of T-helper cells. CD4+ T cells are central to the regulation of immune responses. They help activate B cells, macrophages, and other immune cells, and they produce cytokines that influence the type of immune response (e.g., TH1, TH2, Tfh, Tregs).
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-CD4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
