BCL6 titration and inhibitor IC50 determination
The BCL6 activity was detected using TR-FRET technology. The reaction was performed by incubating the BCL6 protein, substrate and the beads at 25℃ for 60 min, then reading ratio 520/490 with BMG.
BCL6 Protein, Human
BCL6 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | B cell CLL/lymphoma 6; B cell lymphoma 6 protein; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein; BCL 5; Bcl 6; BCL-5; BCL-6; BCL5; BCL6; BCL6_HUMAN; BCL6A; Protein LAZ-3; ZBTB 27; ZBTB27; Zinc finger protein 51; zinc finger transcription factor BCL6S; ZNF 51; ZNF51 |
| Accession | P41182-1 |
| Amino Acid Sequence |
MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG HGSHHHHHHS GLEVLFQGPA DSCIQFTRHA SDVLLNLNRL RSRDILTDVV IVVSREQFRA HKTVLMACSG LFYSIFTDQL KCNLSVINLD PEINPEGFCI LLDFMYTSRL NLREGNIMAV MATAMYLQME HVVDTCRKFI KASE |
| Expression System | E.coli |
| Molecular Weight | 30.4 kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg BCL6
1 3 μg BCL6
