BCL6 titration and inhibitor IC50 determination
The BCL6 activity was detected using TR-FRET technology. The reaction was performed by incubating the BCL6 protein, substrate and the beads at 25℃ for 60 min, then reading ratio 520/490 with BMG.
| Species | Human |
| Synonyms | B cell CLL/lymphoma 6,B cell lymphoma 6 protein,B-cell lymphoma 5 protein,B-cell lymphoma 6 protein,BCL 5,Bcl 6,BCL-5,BCL-6,BCL5,BCL6,BCL6_HUMAN,BCL6A,Protein LAZ-3,ZBTB 27,ZBTB27,Zinc finger protein 51,zinc finger transcription factor BCL6S,ZNF 51,ZNF51 |
| Accession | P41182-1 |
| Amino Acid Sequence | A5-E129,N-terminal Trx and 6x His tag |
| Expression System | E.coli |
| Molecular Weight | 30.4 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |

BCL6 titration and inhibitor IC50 determination
The BCL6 activity was detected using TR-FRET technology. The reaction was performed by incubating the BCL6 protein, substrate and the beads at 25℃ for 60 min, then reading ratio 520/490 with BMG.

M MW Standard
1 1 μg BCL6
1 3 μg BCL6
