WB result of ASGR1 Rabbit pAb
Primary antibody: ASGR1 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 50 kDa
ASGR1 Rabbit Polyclonal Antibody
ASGR1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ASGR1 |
| Synonyms | Asialoglycoprotein receptor 1; ASGP-R 1; C-type lectin domain family 4 member H1; ASGPR 1; Hepatic lectin H1 (HL-1); CLEC4H1 |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | P07306 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HepG2 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
The ASGR1 protein, also known as the asialoglycoprotein receptor 1, is a key component of the asialoglycoprotein receptor complex, which is involved in the recognition and uptake of glycoproteins with terminal galactose or N-acetylgalactosamine residues. This receptor is primarily expressed in hepatocytes and plays a crucial role in the clearance of circulating glycoproteins, contributing to the maintenance of homeostasis. The ASGR1 gene is located on chromosome 17 and encodes a 46 kDa glycoprotein that forms a complex with the ASGR2 subunit. Recent studies have also highlighted the potential role of ASGR1 in various metabolic processes and its implications in diseases such as atherosclerosis.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human placenta. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
