Flow cytometric analysis of Human CD45 expression on human PBMC (human peripheral blood mononuclear cell). human PBMC were stained with either APC-Cy7 Mouse IgG1, κ Isotype Control (Black line histogram) or SDT APC-Cy7 Mouse Anti-Human CD45 Antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC-Cy7 Mouse Anti-Human CD45 Antibody (S-839-3)
APC-Cy7 Mouse Anti-Human CD45 Antibody (S-839-3)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD45 |
| Synonyms | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P08575 |
| Clone Number | S-839-3 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 0.1mg/ml |
| Conjugation | APC-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 2.5 μl per million cells in 100μl volume |
Background
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
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