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Red Blood Cell Lysis Buffer (Contains no fixative,1X)

Red Blood Cell Lysis Buffer (Contains no fixative,1X)

Catalog Number: S0D9002 Brand: Starter
Price:
Regular price $20 USD
Regular price Sale price $20 USD
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Product Details

Product Specification


Conjugation Unconjugated
Physical Appearance Liquid
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Background

This product, Red Blood Cell Lysis Buffer, is a solution designed to lyse and remove anucleated red blood cells from fresh blood or tissue samples derived from humans, rats, mice, and other species. Through optimized formulation, it lyses red blood cells while causing virtually no damage to lymphocytes or other nucleated cells. Under normal conditions, more than 80% of the total white blood cells in the sample can be recovered using this product, with cell viability fully preserved. The lysis buffer has undergone sterile treatment, and the isolated cell samples can be directly used for subsequent primary cell culture, cell fusion, flow cytometry sorting, as well as direct extraction of nucleic acids or proteins and various routine analyses and assays. The main component of this lysis buffer is ammonium chloride, and it contains no fixatives.

Picture

Contents & Protocol

在使用前请平衡红细胞裂解液至室温;

对于组织细胞样品:
1. 新鲜组织经过胶原酶或胰酶等消化处理,通过适当方法分散成细胞悬液,1500rpm离心3分钟,弃上清。
2. 加入3-5倍细胞体积的红细胞裂解液,轻轻吹打混匀,室温裂解3分钟。
3. 加入同等体积的缓冲液(如PBS,pH 7.2~7.4),4℃ 3000rpm离心5min,弃红色上清。
4. 如果发现红细胞裂解不完全,可以重复上述步骤2和步骤3一次。通常极微量的红细胞不会影响后续实验。
5. 用适当的缓冲液清洗1~2次。重悬沉淀,4ºC 1500rpm 离心2-3分钟,弃上清。
6. 用适当溶液重悬细胞沉淀后即可进行后续的操作。

对于血液样品:
1. 取新鲜抗凝血,加入5-10倍细胞体积的红细胞裂解液,轻轻吹打混匀,室温裂解3-5分钟。
2. 加入同等体积的缓冲液(如PBS,pH 7.2~7.4),4℃ 3000rpm离心5min,弃红色上清。
3. 如果发现红细胞裂解不完全,可以重复上述步骤2和步骤3一次。通常极微量的红细胞不会影响后续实验。
4. 用适当的缓冲液清洗1~2次。重悬沉淀,4ºC 1500rpm 离心2-3分钟,弃上清。
5. 用适当溶液重悬细胞沉淀后即可进行后续的操作。