• Rat TNF-α ELISA Kit
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Rat TNF-α ELISA Kit

Rat TNF-α ELISA Kit

Catalog Number: abs530007 Application: ELISA Reactivity: Rat Conjugation:
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$450 USD
$450 USD
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Product Details

Product Specification

Species Reactivity Rat
Theory This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat TNF-α antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).
Composition Please use it within the validity period of the kit (new and old products are shipped randomly)
Component Specifications Shelf life of diluted or redissolved reagent after unpacking
Rat TNF-α Microplate 1 After the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day
Rat TNF-α Standard 2 After dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day
Rat TNF-α Detection antibodies 1 After the concentrated volume is dissolved, it can be in 2-8°C store 14 Day
40×SA-HRP 1 40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage
10× Buffer for concentration and dilution 1 After opening, it can be found in 2-8°C store 30 Day
Chromogenic liquid 1
Stop liquid 1
20× Concentrated Wash Buffer 1
Sealing film 3 Stored at room temperature, to avoid contamination, not reusable
Background Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototype ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-α is also implicated in a number of pathological conditions, including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmune diseases, and cancer. Rat TNF-alpha is a 26kDa type II transmembrane protein, which consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane fragment, and a 156 aa extracellular domain (ECD). In ECD, rat TNF-alpha has 95% amino acid sequence identity with mouse and 73%-79% amino acid sequence identity with bovine, canine, cotton rat, horse, cat, human, macaque, and pig TNF-alpha. It is produced by a variety of immune cells, epithelial cells, endothelial cells and tumor cells. TNF-α assembles intracellularly to form non-covalently linked homotrimers expressed on the cell surface. Cell surface TNF-α can not only induce lysis of tumor cells and virus-infected cells, but also produce its own downstream cell signaling after ligation through soluble TNFRI. TACE/ADAM17 releases membrane-bound TNF-α to release a bioactive cytokine, which is a 55 kDa soluble trimer of the extracellular domain of TNF-α. TNF-α binds to the ubiquitous 55-60 kDa TNFRI and the hematopoietic cell-restricted 78-80 kDa TNFRII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-α with comparable affinity and can promote NFκB activation. However, only TNF RI contains a cytoplasmic death domain, which triggers the activation of apoptosis. Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-α.
General Notes 1. Please use the kit within the validity period.
2. The components of different kits and different batch kits cannot be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent (1 ×) in the last step.
4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.
Storage Temp. Kit unopened, stored at 2-8 °C.
Test Range 62.5pg/mL-4000pg/mL