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Rat IL-2 ELISA Kit

Rat IL-2 ELISA Kit

Catalog Number: abs530005 Application: ELISA Reactivity: Rat Conjugation:
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$450 USD
$450 USD
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Product Details

Product Specification

Theory Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-rat IL-2 antibody was precoated on a high-affinity microplate. Enzyme label plate hole with standard substance, the sample under test and biotinylated antibodies detection, after incubation, IL - 2 that exist in the sample combined with solid phase antibody and detection antibody, immune complex. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.
Composition
Component Size Once opened, diluted or heavy soluble reagent expiration date
Rat  IL-2 Microplate 1 Unused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants
Rat  IL-2 standard substance 2 Dissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days
Rat  IL-2 detect antibody 1 Enrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days
40×SA-HRP 1 40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save
10 x condense dilute with buffer 1 After opening, can be in 2-8 ° C stored for 30 days
Color-substrate solution 1
Stop buffer 1
20 x inspissation washing buffer 1
Sealing film 3 Room temperature storage, to avoid contamination, do not repeat use
Background Interleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. "Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor." Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25/IL-2Rα+Treg cells. Notably, IL-2 is now recognized as essential for successful induction of recall responses in CD8+ memory cells. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. Two of these subunits can bind ligands and are termed IL-2Rα and IL-2Rβ. IL-2Rα is 55kDa and binds IL-2 with low affinity. Il-1β, 75kDa, is also a component of the IL-15 receptor and binds IL-2 with moderate affinity. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). γc shares receptors with IL-4, -7, -9, -15 and -21. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R.
General Notes 1. Please use the kit within the validity period.
2. Components of different kits and different batch kits should not be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. "If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used."
4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.
Storage Temp. Unopened kit, 2-8° C Storage
Test Range 62.5pg/mL-4000pg/mL

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