Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is less than 6ng/ml.
RANK L/TNFSF11(182aa) Protein, Human
RANK L/TNFSF11(182aa) Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Tumor necrosis factor ligand superfamily member 11 (TNFSF11); Osteoclast differentiation factor (ODF); Osteoprotegerin ligand (OPGL); Receptor activator of nuclear factor kappa-B ligand (RANKL); TNF-related activation-induced cytokine (TRANCE); |
| Accession | O14788 |
| Amino Acid Sequence | Gly64-Asp245 |
| Expression System | HEK293 |
| Molecular Weight | 25-33 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 100mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Front Immunol. 2014 Oct 20:5:511. eCollection 2014. |
Background
TNFSF11, also known as RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand), is a cytokine belonging to the tumor necrosis factor (TNF) superfamily. It plays a crucial role in regulating bone metabolism and immune responses. TNFSF11 is primarily expressed by osteoblasts and activated T cells and functions as a key mediator of osteoclast differentiation and activation. By binding to its receptor RANK (Receptor Activator of Nuclear Factor Kappa-B) on osteoclast precursor cells, TNFSF11 stimulates osteoclastogenesis and bone resorption. Additionally, TNFSF11 is involved in the regulation of immune responses, lymph node development, and mammary gland development.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
