Flow cytometric analysis of NHP PBMC labeling HLA-DR PerCP-Cy5.5 Isotype after incubated for 30 min on ice in PBS (Blue) compared with NHP FcR Blocking Reagent at 3 µg/test (Red) to block Fc receptors and non-specific protein. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
| Application | Fc receptor blocking |
| Reactivity | Mk |
| Concentration | 3 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| Fc receptor blocking | 3μg (1μl/test) per million cells in 100μl volume |
Background
FcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.
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