Mouse IL-1β ELISA Kit

Catalog Number: abs520001 Application: ELISA Reactivity: Mouse Conjugation:

Price:

$450 USD

Size: 96T

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Theory

Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-1β antibody was precoated on a high-affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-1β present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).

Composition

Component	Size	Once opened, diluted or heavy soluble reagent expiration date
Mouse IL-1β Microplate	1	Unused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants
Mouse IL-1β standard substance	2	Dissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days
Mouse IL-1β detect antibody	1	Enrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days
40×SA-HRP	1	40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save
10 x condense dilute with buffer	1	After opening, can be in 2-8 ° C stored for 30 days
Color-substrate solution	1
Stop buffer	1
20 x inspissation washing buffer	1
Sealing film	3	Room temperature storage, to avoid contamination, do not repeat use

Background

The interleukin-1 (IL-1) protein family contains the classical members IL-1α, Il-1β and IL-1ra, as well as IL-18, IL-33 and IL-1F5-10. IL-1α and IL-1β bind to the same cell surface receptor and share biological functions. "With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, unstimulated cells from healthy mice do not produce IL-1." "However, in response to inflammatory agents, infections, or microbial endotoxins, IL-1 is produced in large quantities by macrophages and a variety of other cells." IL-1β plays a key role in the physiological processes of immune response and inflammation, bone remodeling, fever, glucose metabolism, growth hormone /IGF-1. "Abnormal or sustained production of IL-1 has been associated with a range of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury, and aging-related diseases."
IL-1α and IL-1β have structurally related peptides that share approximately 25% identity at the amino acid level. They were synthesized as the size of 31 kDa precursor, and then cracking into mature protein, molecular weight is about 17.5 kDa. Caspase 1 / ICEI cracking IL - 1 beta precursor is a key step in the inflammatory response. Neither IL-1α nor IL-1β contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through nonclassical pathways. Part of raw IL - 1 alpha can appear on the cell membrane, and may have biological activities. Unlike precursor IL - 1 alpha, beta IL - 1 precursor compared with mature body almost do not have biological activities. Both unprocessed and mature IL-1β can be exported from the cell.
IL - 1 alpha and beta IL - 1 with IL - 1 ra combination immunoglobulin superfamily receptors play a role. "Cells expressing the 80-kda transmembrane type I receptor (IL-1RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid lining cells, chondrocytes, and hepatocytes." "Cells expressing the 68kDa transmembrane type II receptor (IL-1RII) include B cells, neutrophils, and bone marrow cells." "The two IL-1 receptors share approximately 28% identity in their extracellular domains, but a significant difference is found in their intracellular domains: the type II receptor has an intracellular domain of only 29 amino acids, whereas the type I receptor has an intracellular domain of 217 amino acids." IL-1RII does not produce a signal for IL-1 and may act as a decoy receptor to attenuate IL-1 function. IL-1 receptor accessory protein (IL-1RAcP) is associated with IL-1RI and required for IL-1RI signal transduction. IL-1ra is a secretory molecule that acts as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1RII can be detected in human plasma, joint synovial fluid, and conditioned media of a variety of human cell lines. In addition, IL-1binding proteins similar to soluble IL-1RII are capable of being encoded by cowpox and vaccinia virus.

General Notes

1. Please use the kit within the validity period.
2. Components of different kits and different batch kits should not be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1&times) is used. "In addition to dilution, cell culture medium may be used for other intermediate dilutions."
4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.

Storage Temp.

Unopened kit, 2-8° C Storage

Test Range

15.6pg/mL-1000pg/mL

Item added to your cart

Mouse IL-1β ELISA Kit