Flow cytometric analysis of Lamin A/C expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) or Jurkat (Human T cell leukemia T lymphocyte, Left) was stained with Phycoerythrin Rabbit IgG Isotype Control (Black line histogram) or SDT Lamin A/C Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) Lamin A/C Recombinant Rabbit mAb (PE Conjugate) (Red line histogram) at 1/1000 dilution (0.1 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Lamin A/C Recombinant Rabbit mAb (PE Conjugate) (SDT-R041)
Lamin A/C Recombinant Rabbit mAb (PE Conjugate) (SDT-R041)
Price:
Regular price
$45 USD
Regular price
Sale price
$45 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lamin A/C |
| Synonyms | Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32 |
| Accession | P02545 |
| Clone Number | SDT-R041 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:1000 | Hu |
Background
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome.
Picture
Picture
FC
