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CRBN/DDB1 Complex His Tag Protein, Human

CRBN/DDB1 Complex His Tag Protein, Human

Catalog Number: UA085063 Brand: UA BIOSCIENCE
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$335 USD
$335 USD
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Product Details

Product Specification


Species Human
Synonyms CRBN/DDB1
Accession Q96SW2、Q16531
Amino Acid Sequence

Met1-Leu442(CRBN) with His Tag at the N-Terminus;
Met1-His1140(DDB1) with His Tag at the N-Terminus
Molecular Weight

55-70kDa&100-130kDa (Reducing)

Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Liquid
Storage Buffer

50mM Tris, 150mM NaCl, pH7.5, 1mM DTT, 10%Glycerol
Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
Reference

1. Fischer, E.S., et al. (2014). Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. Nature, 512(7512), 49-53.
2. Darling, S., et al. (2025). From serendipity to strategy: molecular glue degraders in cancer therapeutics. Critical Reviews in Biochemistry and Molecular Biology, 60(4-6), 200-230.
3. Kroupova, A., et al. (2024). Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders. Nature Communications, 15(1), 8885.

Picture

Bioactivity

Activity assay of CRBN/DDB1 His Tag Protein, Human (Cat.No.UA085063) via TR-FRET technology:Incubate CRBN/DDB1 His Tag Protein, Human, substrate, corresponding donor and acceptor at 25 °C for 120 min. Finally, read the ratio of fluorescence signals at 620 nm / 665 nm on a TMO Varioskan LUX.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).