The cRAF activity was detected using HTRF technology. The reaction was performed by incubating the cRAF protein, GTP, KRAS[G12C] and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
cRAF, GST Tag Protein
cRAF, GST Tag Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | c-Raf, CMD1NN, CRAF, NS5, Raf-1 |
| Accession | P04049 |
| Amino Acid Sequence | M1-L149 |
| Expression System | E.coli |
| Molecular Weight | 43094.0 |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.4 ,10% glycerol |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg cRAF
2 3 μg cRAF
HTRF
