2μg (R: reducing condition, N: non-reducing condition).
ACSS1 His Tag Protein, Human
ACSS1 His Tag Protein, Human
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$200 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | ACSS1, ACAS2L, KIAA1846, Acetate--CoA ligase 2 |
| Accession | Q9NUB1 |
| Amino Acid Sequence | Ala38-Lys689 with His Tag at the N-Terminus |
| Expression System | E.coli |
| Molecular Weight | 70-100kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, pH7.5, 1mM DTT, 10%Glycerol |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
| Reference | 1. Fu, X., et al. (2025). ACSS1 co-opts acetyl-CoA metabolism to drive DNA repair and undermine radiotherapy efficacy in breast cancer. Cell Death & Disease, 17, 119. |
Background
ACSS1 (Acyl-CoA Synthetase Short-Chain Family Member 1) is a key metabolic enzyme localized in the mitochondrial matrix. Encoded by the human ACSS1 gene on chromosome 20p11.21, the protein is 689 amino acids long and features an N-terminal acetyl-CoA synthetase domain and two AMP-binding domains.
Functionally, ACSS1 catalyzes the ATP-dependent conversion of acetate and CoA into acetyl-CoA, a critical step in mitochondrial energy metabolism that supplies the TCA cycle. Under glucose deprivation, ACSS1 utilizes alternative carbon sources like acetate to sustain energy production in high-energy organs such as the heart and brain. Its enzymatic activity is regulated by SIRT3-mediated deacetylation at a key lysine residue (K642 in humans).
Dysregulation of ACSS1 expression and function is implicated in various diseases. In oncology, ACSS1 is aberrantly overexpressed in breast cancer, AML, and melanoma, where it rewires acetyl-CoA metabolism to drive DNA repair, promote tumor growth and metastasis, and is associated with poor prognosis and therapy resistance. Furthermore, reduced ACSS1 function may contribute to energy failure in neurodegenerative diseases and is linked to metabolic disorders including nonalcoholic fatty liver disease and sarcopenia.
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SDS-PAGE
SEC-HPLC
The purity of ACSS1 His Tag Protein, Human is more than 95% determined by SEC-HPLC.
