WB result of V5 Tag (HRP Conjugate) Mouse mAb
Primary antibody: V5 Tag (HRP Conjugate) Mouse mAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with Histone H3-V5 tag fusion protein whole cell lysate 20 µg
Predicted MW: 17 kDa
Observed MW: 17 kDa
V5 Tag Mouse mAb (HRP Conjugate) (S-610-66)
V5 Tag Mouse mAb (HRP Conjugate) (S-610-66)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Synthetic Peptide |
| Clone Number | S-610-66 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000 |
Background
V5-tag is a short peptide tag for detection and purification of proteins. The V5-tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and nanobodies.
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Picture
Western Blot
