WB result of E tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: E-tagged fusion protein
Primary antibody:
Lane 1: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 26 kDa
Observed MW: 26 kDa
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | E tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-911-14 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Species Independent |
Background
The E tag is an epitope tag with a 13-residue sequence of GAPVPYPDPLEPR. The E tag can help with detection of proteins in western blot, IP, or microscopic techniques.
Picture
Picture
Western Blot
