WB result of E tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: E-tagged fusion protein
Primary antibody:
Lane 1: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: E tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 26 kDa
Observed MW: 26 kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | E tag |
Immunogen | Synthetic Peptide |
Clone Number | S-911-14 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | WB |
Reactivity | Species Independent |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | HRP |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
application | dilution | species |
WB | 1:5000-1:50000 | Species Independent |
Background
The E tag is an epitope tag with a 13-residue sequence of GAPVPYPDPLEPR. The E tag can help with detection of proteins in western blot, IP, or microscopic techniques.
Picture
Picture
Western Blot
