Rabbit Anti-HA Agarose Beads is used to immunoprecipitate HA-tagged fusion proteins from Escherichia coli lysate supernatant. During elution with Peptide elution buffer, the enriched HA-tagged fusion proteins are released from the Agarose Beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: Flow-through
Lane 4: HA-tagged fusion protein
Lane 5: HA-tagged fusion protein
Product Details
Product Details
Product Specification
Host | Rabbit |
Immunogen | Recombinant Protein |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | IP, Protein purification, ChIP |
Purification | Protein A |
Conjugation | Agarose beads |
Physical Appearance | Liquid |
Storage Buffer | 50% slurry in PBS pH7.4, 0.05% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Rabbit Anti-HA Agarose Beads is an affinity chromatography medium designed for easy, one-step purification of recombinant HA tag fusion proteins in E. coli, insect cells and mammalian cells. The recombinant HA tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-HA Agarose Beads. It is the excellent choice for high performance purifications.
Picture
Picture
SDS-PAGE
