Rabbit Anti -GST agarose Beads were used to immunoprecipitate GST (glutathione S-transferase) from E.coli system.
During elution with Acidic elution buffer buffer, the enriched GST fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: RFP fusion protein.
Product Details
Product Details
Product Specification
Host | Rabbit |
Immunogen | Recombinant Protein |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | IP, Protein purification, Co-IP |
Reactivity | Species Independent |
Purification | Protein A |
Conjugation | Agarose beads |
Physical Appearance | Liquid |
Storage Buffer | 50% slurry in PBS pH7.4, 0.05% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Rabbit Anti-GST agarose Beads is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-GST agarose Beads. It is the excellent choice for high performance purifications.
Picture
Picture
SDS-PAGE
