| Host | Rabbit |
| Antigen | 2-hydroxyisobutyryllysine |
| Synonyms | Khib, Pan 2ohibu |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
| application | dilution | species |
| WB | 1:500 |
Lysine 2-hydroxyisobutyrylation (Khib) is a newly identified post-translational modification (PTM) that involves the attachment of a 2-hydroxyisobutyryl group to lysine residues in proteins. This modification has been found to be widespread across prokaryotic and eukaryotic organisms, including its discovery in plants. It plays a broad regulatory role in cellular processes, including protein biosynthesis and central carbon metabolism.
WB result of 2-hydroxyisobutyryllysine Rabbit pAb
Primary antibody: 2-hydroxyisobutyryllysine Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml 2-Hydroxyisobutyric acid for 18 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
