The STAT6 activity was detected using TR-FRET technology. The reaction was performed by incubating the varying concentrations of SI-109 and STAT6 protein at 25℃ for 10 min, adding substrate and the beads at 25℃ for 60 min, then reading ratio 520/485 with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Accession | P42226-1 |
Amino Acid Sequence | W123-T658; N-terminal 6x His tag |
Expression System | E.coli |
Molecular Weight | 62.0 kDa |
Purity | >90% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | His Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
