2μg (R: reducing condition).
SKP1 His Tag Protein, Human
SKP1 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | EMC19,OCP2,SKP1A,TCEB1L |
| Accession | P63208 |
| Amino Acid Sequence | Met1-Lys163 with His Tag at the N-Terminus |
| Expression System | E.coli |
| Molecular Weight | 21kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Schulman BA, Carrano AC, Jeffrey PD, et al. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature. 2000;408(6810):381-386. |
Background
SKP1 (S-phase kinase-associated protein 1) is a core adaptor subunit of the SCF (SKP1-Cullin1-F-box) class of E3 ubiquitin ligases. By bridging the Cullin-1 scaffold to variable F-box proteins, SKP1 determines which substrates are poly-ubiquitylated and subsequently degraded by the 26 S proteasome. In this way SKP1 controls eukaryotic cell-cycle transitions, signal transduction, transcription, circadian rhythms and stress responses.
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SDS-PAGE
