1μg (R: reducing condition, N: non-reducing condition).
Siglec-3 (R69G) His Tag Protein, Human
Siglec-3 (R69G) His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Myeloid Cell Surface Antigen CD33,CD33 Molecule Transcript,CD33 Antigen,Siglec-3,P67,FLJ00391,Sialic acid-binding Ig-like lectin 3,CD33,CD33 Molecule,CD33 Antigen (Gp67),SIGLEC3,Gp67,Sialic Acid Binding Ig-Like Lectin 3 |
| Accession | P20138 |
| Amino Acid Sequence | Asp18-His259 (Arg69Gly) with His Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 43-55kDa (Reducing) |
| Purity | >95% as determined by SDS-PAGE & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1、Ulyanova T. et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9. |
Background
Siglecs are transmembrane proteins of the immunoglobulin superfamily that bind sialic acid. They include an N-terminal V-set Ig-like domain and various C2-set domains. The first characterized Siglecs, including Siglec-1 to -4, show 25–30% sequence identity in their extracellular regions. A cluster of Siglec genes related to CD33 is found on human chromosome 19q13.3-4. The CD33-related subgroup (Siglecs-3, -5, -6, -7, and -8) shares 50–70% sequence identity. Each Siglec has a unique role in hemopoietic cell biology.
CD33 (Siglec-3), the smallest Siglec member, binds α2-6- and α2-3-sialylated glycans and is highly expressed on leukemic cell lines. It's primarily found on myeloid lineage leukocytes but also appears on lymphoid cells, including NK cells. CD33 exists as two membrane isoforms: full-length CD33M and CD33m, which lacks the V extracellular domain. CD33 expression is linked to Alzheimer’s disease, autoimmune conditions like SLE, type II diabetes, and infections.
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SDS-PAGE
SEC-HPLC
The purity of Siglec-3 (R69G) His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized Siglec-3 (R69G) His Tag Protein, Human (Cat. No. UA011232) at 2.0μg/mL (100μL/well) can bind Anti-Human SIGLEC3 (Gemtuzumab) with EC50 of 2.64-4.99ng/mL.
