Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical EC50 is less than 10ng/ml in the presence of 5ng/ml Recombinant Human Wnt Surrogate.
R-Spondin2/RSPO2 Protein, Mouse
R-Spondin2/RSPO2 Protein, Mouse
Price:
Regular price
$164 USD
Regular price
Sale price
$164 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | R-spondin-2,Cysteine-rich and single thrombospondin domain-containing protein 2 (Cristin-2,mCristin-2),Roof plate-specific spondin-2 |
| Accession | Q8BFU0 |
| Amino Acid Sequence | Ala32-Gly205, with N-terminal hIgG1 Fc Tag |
| Expression System | HEK293 |
| Molecular Weight | 46-55 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Development. 2008 Mar;135(6):1049-58. |
Background
R-Spondin2 (RSPO2) is a member of the R-Spondin family and is a secreted protein that plays a crucial role in regulating Wnt/β-catenin signaling. In mice, R-Spondin2 is essential for various developmental processes. For example, it is required for the maintenance of the apical ectodermal ridge in the hindlimb, which is critical for proper limb development. Additionally, R-Spondin2 is involved in the development of the larynx, trachea, lung, and other organs. It also plays a role in bone formation and mineralization by enhancing Wnt/β-catenin signaling.
R-Spondin2's ability to enhance Wnt signaling makes it a potential candidate for promoting tissue regeneration and repair. It can be used to stimulate osteoblast differentiation and bone formation, which is beneficial for treating bone-related disorders. Also, R-Spondin2 is involved in the regulation of stem cell proliferation and differentiation. Its role in Wnt signaling can be exploited to study cancer stem cells and develop targeted therapies for cancers that involve dysregulated Wnt pathways
Picture
Picture
Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
RP-HPLC
>95% as determined by RP-HPLC.
