pHAb Thiol Reactive Dye
pHAb Thiol Reactive Dye
Product Details
Product Details
Product Specification
| Physical Appearance | Lyophilized Powder |
| Stability & Storage | The dyes can be stored at -20°C for up to one month. For long-term storage, they should be kept below -65°C. Avoid repeated freezing and thawing. |
Background
pHAb Dyes are pH sensor dyes that have very low fluorescence at pH > 7 and a dramatic increase in fluorescence as the pH of the solution becomes acidic. pHAb Dyes have excitation maxima (Ex) at 532nm and emission maxima (Em) at 560nm.
pHAb Reactive Dyes are designed specifically for antibody labeling and can be used for high-throughput antibody internalization screening assays.
pHAb Thiol Reactive Dye has a maleimide group that reacts with thiols. This maleimide group is conjugated to the antibody after the cysteine disulfide bonds in the hinge region of the antibody are reduced to thiols through the use of a reducing agent, such as DTT or TCEP. pHAb Reactive Dyes retain their fluorescent response to decreases in pH even after conjugation to antibody.
Here are some recommended instrument parameters:
• Microplate Reader: Tecan Infinite® M1000 Pro (Ex: 532 nm / Em: 560 nm)
• Flow Cytometer: Millipore Guava easyCyte 8HT, using yellow fluorescence parameters (Em filter: 583/26)
• Fluorescence Microscope: Olympus FV 500; TAMRA setting (Ex: 543 nm / Em: 565 nm)
• Fluorescence Imaging System: Life Technologies EVOS® FL Cell Imaging System, RFP red cube filter set (Ex: 531 nm / Em: 593 nm)
• Fluorescence Gel Scanner: GE Typhoon® 9410; TAMRA setting (Ex: 532 nm / Em: 580 nm)
