Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The EC50 for this effect is 9.3-27 ng/mL.
IL-36α Protein, Mouse
IL-36α Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Interleukin-36 alpha; FIL1 epsilon; Interleukin-1 epsilon (IL-1 epsilon); Interleukin-1 family member 6 (IL-1F6); Interleukin-1 homolog 1 (IL-1H1); |
| Accession | Q9JLA2 |
| Amino Acid Sequence | Arg8-His160 |
| Expression System | E.coli |
| Molecular Weight | 17 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 50mM NaCl, 1mM DTT, 5% trehalose, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | J Leukoc Biol. 2015 Apr;97(4):645-52. |
Background
Interleukin-36α (IL-36α, formerly IL-1F6) is a pro-inflammatory member of the IL-1 super-family. The mature mouse protein is an 18 kDa, 160-amino-acid, β-trefoil monomer that lacks a signal peptide and glycosylation sites. Full-length IL-36α is only weakly active; removal of the N-terminal methionine by neutrophil proteases (cathepsin G, elastase, proteinase-3) generates the highly active form. In mice, IL-36α mRNA is constitutively detected in skin keratinocytes, stomach, trachea, fetal brain, spleen, lymph nodes, kidney tubules and adipose tissue, and is rapidly up-regulated after TLR4 ligation, IL-1β, TNF-α or fungal challenge. Once released (passively during necrosis or through P2X7-dependent externalisation), IL-36α binds the heterodimeric receptor IL-1Rrp2/IL-1RAcP expressed on keratinocytes, dendritic cells, macrophages and CD4⁺ T cells. Engagement activates MyD88 → NF-κB and MAPKs (p38, JNK, ERK), leading to transcription of IL-6, IL-23, TNF-α, CXCL1, CXCL2, CCL3, β-defensin-3 and GM-CSF, and thereby amplifies Th1/Th17 immunity while recruiting neutrophils and monocytes.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
RP-HPLC
>95% as determined by RP-HPLC.
