IL-33 Protein, Human
IL-33 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Interleukin-33,IL-1 F11,NF-HEV |
| Accession | O95760 |
| Amino Acid Sequence | Ser112-Thr270 |
| Expression System | E.coli |
| Molecular Weight | Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids. |
| Purity | ＞96% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20 mM PB, with 150 mM NaCl, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20% a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, Peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58% a.a. sequence identity with mouse and rat IL-33. Recombinant Human IL-33 is a 17.9kDa protein containing 159 amino acid residues polypeptide chain.
