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Human CD6, His tag

Human CD6, His tag

Catalog Number: S0A1037 Brand: Starter
Price:
Regular price $100.00 USD
Regular price Sale price $100.00 USD
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Product Details

Product Specification


Species Human
Synonyms T12, TP120
Accession P30203
Amino Acid Sequence

Protein sequence(P30203, His18-Glu398, with C-10*His) HPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLCSQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESREGGGGSHHHHHHHHHH

Expression System HEK293
Molecular Weight Theoretical:42.2kDa Actual:90kDa
Purity

>95% by SDS-PAGE

Endotoxin <1EU/μg
Tag with C-10*His
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

Background

CD6 is a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. This protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. It is important for continuation of T cell activation.

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Bioactivity

Immobilized Human CD6, His tag at 2 μg/mL (50 μL/well) can bind Recombinant Human ALCAM/CD166 Protein with EC50 of 16.38-23.59 ng/ml.

SDS-PAGE

2μg(R: reducing conditions)

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