Immobilized Human AFP, His tag at 0.5 μg/mL (50 μL/well) can bind AFP Recombinant Rabbit mAb (SDT-R077) (S0B2095) with EC50 of 4.091-4.842 ng/mL.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP |
Accession | P02771 |
Amino Acid Sequence | Protein sequence(P02771, Met1-Val609, with C-10*His) |
Expression System | HEK293 |
Molecular Weight | Theoretical:68.1kDa Actual:72kDa |
Purity | >90% by SDS-PAGE |
Endotoxin | <1EU/μg |
Tag | His Tag |
Physical Appearance | Lyophilized Powder |
Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles. |
Background
AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal development. It is thought to be the fetal analog of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. Measurement of AFP is generally used in two clinical contexts. First, it is measured in pregnant women through the analysis of maternal blood or amniotic fluid as a screening test for certain developmental abnormalities, such as aneuploidy. Second, serum AFP level is elevated in people with certain tumors.
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Bioactivity
SDS-PAGE

2μg(R: reducing conditions)

