HRAS enzyme titration
Product Details
Product Details
Product Specification
Species | Human | |
Synonyms | C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, p21ras, RASH1 | |
Accession | P01112-1 | |
Amino Acid Sequence |
M1-H166 MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQH |
|
Expression System | E.coli | |
Molecular Weight | 22.9 kDa | |
Purity | > 95% by SDS-PAGE | |
Endotoxin | <1EU/μg | |
Conjugation | Unconjugated | |
Tag | Avi Tag, His Tag | |
Physical Appearance | Liquid | |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 | |
Stability & Storage |
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Bioactivity


The HRAS activity was detected using HTRF technology. The reaction was performed by incubating the HRAS protein,
substrate and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.



