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GFRAL Fc Chimera Protein, Human

GFRAL Fc Chimera Protein, Human

Catalog Number: UA011250 Brand: UA BIOSCIENCE
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$281.00 SGD
$281.00 SGD
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Product Details

Product Specification


Species Human
Synonyms GFR alpha like,GRAL,GDNF family receptor alpha-like
Accession Q6UXV0
Amino Acid Sequence

Ser19-Glu351 with Human IgG1 Fc at the C-Terminus
Expression System HEK293
Molecular Weight

60-80kDa (Reducing)
Purity ＞95% by SDS-PAGE &SEC-HPLC
Conjugation Unconjugated
Tag Human IgG1 Fc
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. 
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.  
· Please avoid repeated freeze-thaw cycles.
Reference

1.Airaksinen MS, Holm L, Hätinen T. Evolution of the GDNF family ligands and receptors. Brain Behav Evol. 2006;68(3):181-90.
2.Emmerson PJ, Wang F, Du Y, Liu Q, Pickard RT, Gonciarz MD, Coskun T, Hamang MJ, Sindelar DK, Ballman KK, Foltz LA, Muppidi A, Alsina-Fernandez J, Barnard GC, Tang JX, Liu X, Mao X, Siegel R, Sloan JH, Mitchell PJ, Zhang BB, Gimeno RE, Shan B, Wu X. The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL. Nat Med. 2017 Oct;23(10):1215-1219.

Background

GFR alpha-like is also known as GDNF family receptor alpha-like, GFRAL, C6orf144, UNQ9356, PRO34128. Macrophage inhibitory cytokine-1/growth differentiation factor 15 (MIC-1/GDF15) is a divergent transforming growth factor (TGFbeta) superfamily cytokine implicated in biological and disease processes including metabolism, cancer, and chronic inflammation. GDNF family receptor α-like (GFRAL) was recently identified as the neuronal brainstem receptor responsible for mediating the anorectic actions of GDF15. Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses . Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.

Picture

SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of GFRAL Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.

ELISA

Immobilized GDF-15 Protein, Mouse (Cat. No.UA040185)
at 2.0μg/mL (100μL/well) can bind GFRAL Fc Chimera
Protein, Human (UA011250)with EC50 of 11.85-19.23
ng/mL.