2μg (R: reducing condition, N:non-reducing condition).
Product Details
Product Details
Product Specification
| Synonyms | E2 |
| Accession | A5Y7Y5 |
| Amino Acid Sequence | Ser326-Gln666 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 43-55kDa (Reducing) |
| Purity | >95% by SDS-PAGE & > 95% SEC-HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.De la Cruz Borthiry FL, Beltrame JS, Cañumil VA, Scheffer F, Bogetti ME, Cella M, Vermeulen M, Gimenez L, Negri Malbrán M, Repetto EM, Cymeryng C, Leguizamon GF, Franchi AM, Ribeiro ML. Chronic mild maternal stress modulates the immune response at the maternal-fetal interface and affects the growth and mortality of the offspring. Placenta. 2025 Oct 3:S0143-4004(25)00711-8. |
Background
Chikungunya virus (CHIKV) envelope protein 2 (E2) plays a central role in viral pathogenesis. This key surface glycoprotein is responsible for receptor binding and facilitating viral entry into host cells. Structurally, E2 forms heterodimers with the E1 fusion protein, which then assemble into the trimeric spike complexes on the viral surface. Given that E2 harbors the virus's principal neutralizing epitopes, it represents a prime candidate antigen for the rational design of vaccines and serological diagnostic assays
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SDS-PAGE
SEC-HPLC
The purity of E2/Envelope2 His Tag Protein, Chikungunya virus CHIKV is more than 95% determined by SEC-HPLC.
