2μg (R: reducing condition, N:non-reducing condition).
E2/Envelope2 GST&His Tag Protein, Chikungunya virus CHIKV
E2/Envelope2 GST&His Tag Protein, Chikungunya virus CHIKV
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Product Details
Product Details
Product Specification
| Synonyms | E2 |
| Accession | A5Y7Y5 |
| Amino Acid Sequence | Ser326-Gln666 with GST&His Tag at the C-Terminus |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 60-72kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & > 95% SEC-HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag, GST Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.de Lima Cavalcanti TYV, Pereira MR, de Paula SO, Franca RFO. A Review on Chikungunya Virus Epidemiology, Pathogenesis and Current Vaccine Development. Viruses. 2022 May 5;14(5):969. |
Background
Chikungunya virus (CHIKV) envelope protein 2 (E2) plays a central role in viral pathogenesis. This key surface glycoprotein is responsible for receptor binding and facilitating viral entry into host cells. Structurally, E2 forms heterodimers with the E1 fusion protein, which then assemble into the trimeric spike complexes on the viral surface. Given that E2 harbors the virus's principal neutralizing epitopes, it represents a prime candidate antigen for the rational design of vaccines and serological diagnostic assays
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SDS-PAGE
SEC-HPLC
The purity of E2/Envelope2 GST&His Tag Protein, Chikungunya virus CHIKV is more than 95% determined by SEC-HPLC.
