The DYRK1B kinase activity was quantified using the ADP-Glo™ assay. The reaction was performed by incubating the DYRK1B protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
DYRK1B GST&Flag Tag Protein, Human
DYRK1B GST&Flag Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | Q9Y463-1 |
| Amino Acid Sequence | M1-S629(end); N-terminal GST-tag-3C; C-terminal -Flag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 96.9 kDa |
| Purity | >90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | GST Tag, Flag Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
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Bioactivity
SDS-PAGE
