1 μg (R: reducing conditions, N: non-reducing conditions).
Cadherin-1/CDH1 His Tag Protein, Human
Cadherin-1/CDH1 His Tag Protein, Human
Price:
Regular price
$520 USD
Regular price
Sale price
$520 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | Cadherin-1/CDH1 |
| Synonyms | CAM 120/80, Epithelial cadherin (E-cadherin), Uvomorulin |
| Accession | P12830 |
| Amino Acid Sequence | Asp155-Ala709, with C-terminal 10*His DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPAGGGSGGGSHHHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 73-81kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Nelson WJ. Regulation of cell-cell adhesion by the cadherin-catenin complex. Biochem Soc Trans. 2008 Apr;36(Pt 2):149-55. |
Background
CDH1 (also known as epithelial cadherin (E-cadherin), a member of the cadherin- catenin complex, is an example in which pathogenic variants can lead to CLP and/or gastric cancer. The human CDH1 gene consists of 16 exons that span 100 kb. Classical cadherins are a family of single-span transmembrane domain glycoproteins that primarily mediate calcium-dependent cell–cell adhesion, which are differentially expressed throughout the body. Cadherins are single-membrane-spanning proteins with a divergent extracellular domain of five repeats and a conserved cytoplasmic domain. Much experimental evidence suggests that the CDH1 is a tumor suppressor and alterations in its expression are linked with multiple tumor types and its clinicopathological features. Loss of CDH1 is used for the diagnosis and prognosis of epithelial cancers.
Picture
Picture
SDS-PAGE
